| 质粒名称: | CGBE1 (pRZ3885) |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 140252 |
| 存储实验室: | Keith Joung |
|
载体骨架基本信息 |
|
| 载体名称: | CMV |
| 载体出品公司: | pCMV-ABEmax-P2A-EGFP (Addgene #112101) |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | 3400 bp |
| 完整质粒大小: | 9909 bp |
| 载体类型: | 哺乳细胞表达 |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | eUNG-BE4max(R33A)-ΔUGI,bpNLS-UNG(E.coli)-rAPOBEC1(R33A)-nCas9-bpNLS-P2A-EGFP |
| 物种来源: | R. norvegicus (rat); E. coli, S. pyogenes |
| 插入大小: | 6544 bp |
| 基因突变: | R33A in rAPOBEC1, D10A in Cas9 |
| 启动子 | CMV |
| 克隆方法: | Gibson克隆 |
| 5' 测序引物 | CGCAAATGGGCGGTAGGCGTG |
| 3' 测序引物 | TAGAAGGCACAGTCGAGG |
|
文献引用方法 |
|
| 材料和方法部分: | CGBE1 (pRZ3885) was a gift from Keith Joung (Addgene plasmid # 140252 ; http://n2t.net/addgene:140252 ; RRID:Addgene_140252) |
| 参考文献部分: | CRISPR C-to-G base editors for inducing targeted DNA transversions in human cells. Kurt IC, Zhou R, Iyer S, Garcia SP, Miller BR, Langner LM, Grunewald J, Joung JK. Nat Biotechnol. 2020 Jul 20. pii: 10.1038/s41587-020-0609-x. doi: 10.1038/s41587-020-0609-x. 10.1038/s41587-020-0609-x PubMed 32690971 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
质粒图谱.png)
载体简介
载体序列
LOCUS CGBE1 (pRZ3885) 9909 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION CMV promoter expression plasmid for
UNG(E.coli)-rAPOBEC1(R33A)-nCas9-P2A-EGFP.
ACCESSION .
VERSION .
KEYWORDS CGBE1 (pRZ3885)
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 9909)
AUTHORS Kurt IC, Zhou R, Iyer S, Garcia SP, Miller BR, Langner LM, Grunewald
J, Joung JK
TITLE CRISPR C-to-G base editors for inducing targeted DNA transversions
in human cells.
JOURNAL Nat Biotechnol. 2020 Jul 20. pii: 10.1038/s41587-020-0609-x. doi:
10.1038/s41587-020-0609-x.
PUBMED 32690971
REFERENCE 2 (bases 1 to 9909)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..9909
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind 220..239
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 473..490
/label=L4440
/note="L4440 vector, forward primer"
protein_bind 607..628
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 643..673
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 681..697
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 686..708
/label=M13/pUC Reverse
/note="In lacZ gene"
primer_bind 705..721
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 705..721
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
polyA_signal 792..1016
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
primer_bind 1005..1022
/label=BGH-rev
/note="Bovine growth hormone terminator, reverse primer.
Also called BGH reverse"
CDS complement(1045..1062)
/codon_start=1
/product="6xHis affinity tag"
/label=6xHis
/translation="HHHHHH"
CDS complement(1071..1787)
/codon_start=1
/product="enhanced GFP"
/label=EGFP
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
EFVTAAGITLGMDELYK"
primer_bind complement(1113..1134)
/label=EGFP-C
/note="EGFP, forward primer"
primer_bind 1462..1481
/label=EXFP-R
/note="For distinguishing EGFP variants, reverse primer"
primer_bind 1721..1742
/label=EGFP-N
/note="EGFP, reverse primer"
CDS complement(1788..1844)
/codon_start=1
/product="2A peptide from porcine teschovirus-1
polyprotein"
/label=P2A
/note="Eukaryotic ribosomes fail to insert a peptide bond
between the Gly and Pro residues, yielding separate
polypeptides."
/translation="ATNFSLLKQAGDVEENPGP"
CDS complement(1863..1883)
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
CDS complement(1926..6026)
/codon_start=1
/product="nickase mutant of the Cas9 endonuclease from the
Streptococcus pyogenes Type II CRISPR/Cas system"
/label=Cas9(D10A)
/note="generates RNA-guided single strand nicks in DNA due
to the D10A mutation in the RuvC catalytic domain"
/translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
ATLIHQSITGLYETRIDLSQLGGD"
CDS complement(7521..7541)
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
primer_bind complement(7590..7609)
/label=T7
/note="T7 promoter, forward primer"
promoter complement(7591..7609)
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
promoter complement(7651..7854)
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind complement(7680..7700)
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
enhancer 7855..8234
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
primer_bind 8406..8425
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
promoter 8504..8608
/gene="bla"
/label=AmpR promoter
CDS 8609..9469
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(8827..8846)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 9640..319
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
61 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
121 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
181 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
241 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
301 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
361 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
421 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
481 agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc
541 gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa
601 cgcaattaat gtgagttagc tcactcatta ggcaccctag gctttacact ttatgcttcc
661 ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga
721 ccatgattac gccaagctct agctagaggt cgacggtatc gagccccagc tggttctttc
781 cgcctcagaa gccatagagc ccaccgcatc cccagcatgc ctgctattgt cttcccaatc
841 ctcccccttg ctgtcctgcc ccaccccacc ccccagaata gaatgacacc tactcagaca
901 atgcgatgca atttcctcat tttattagga aaggacagtg ggagtggcac cttccagggt
961 caaggaaggc acgggggagg ggcaaacaac agatggctgg caactagaag gcacagtcga
1021 ggctgatcag cgggtttaaa ctcaatggtg atggtgatga tgaccggtca cttgtacagc
1081 tcgtccatgc cgagagtgat cccggcggcg gtcacgaact ccagcaggac catgtgatcg
1141 cgcttctcgt tggggtcttt gctcagggcg gactgggtgc tcaggtagtg gttgtcgggc
1201 agcagcacgg ggccgtcgcc gatgggggtg ttctgctggt agtggtcggc gagctgcacg
1261 ctgccgtcct cgatgttgtg gcggatcttg aagttcacct tgatgccgtt cttctgcttg
1321 tcggccatga tatagacgtt gtggctgttg tagttgtact ccagcttgtg ccccaggatg
1381 ttgccgtcct ccttgaagtc gatgcccttc agctcgatgc ggttcaccag ggtgtcgccc
1441 tcgaacttca cctcggcgcg ggtcttgtag ttgccgtcgt ccttgaagaa gatggtgcgc
1501 tcctggacgt agccttcggg catggcggac ttgaagaagt cgtgctgctt catgtggtcg
1561 gggtagcggc tgaagcactg cacgccgtag gtcagggtgg tcacgagggt gggccagggc
1621 acgggcagct tgccggtggt gcagatgaac ttcagggtca gcttgccgta ggtggcatcg
1681 ccctcgccct cgccggacac gctgaacttg tggccgttta cgtcgccgtc cagctcgacc
1741 aggatgggca ccaccccggt gaacagctcc tcgcccttgc tcaccatcgg tccaggattc
1801 tcttcgacat ctccggcttg tttcagcaga gagaagtttg ttgcgccgct tccaccgcct
1861 ccgactttcc tcttcttctt gggctcgaat tcgctgccgt cggcggttct ttttgagccg
1921 ccagagtcac ctcccagctg agacaggtcg atccgtgtct cgtacaggcc ggtgatgctc
1981 tggtggatca gggtggcgtc cagcacctct ttggtgctgg tgtacctctt ccggtcgatg
2041 gtggtgtcaa agtacttgaa ggcggcaggg gctcccagat tggtcagggt aaacaggtgg
2101 atgatattct cggcctgctc tctgatgggc ttatcccggt gcttgttgta ggcggacagc
2161 actttgtcca gattagcgtc ggccaggatc actctcttgg agaactcgct gatctgctcg
2221 atgatctcgt ccaggtagtg cttgtgctgt tccacaaaca gctgtttctg ctcattatcc
2281 tcgggggagc ccttcagctt ctcatagtgg ctggccaggt acaggaagtt cacatatttg
2341 gagggcaggg ccagttcgtt tcccttctgc agttcgccgg cagaggccag cattctcttc
2401 cggccgtttt ccagctcgaa cagggagtac ttaggcagct tgatgatcag gtcctttttc
2461 acttctttgt agcccttggc ttccagaaag tcgatgggat tcttctcgaa gctgcttctt
2521 tccatgatgg tgatccccag cagctctttc acactcttca gtttcttgga cttgcccttt
2581 tccactttgg ccaccaccag cacagaatag gccacggtgg ggctgtcgaa gccgccgtac
2641 ttcttagggt cccagtcctt ctttctggcg atcagcttat cgctgttcct cttgggcagg
2701 atagactctt tgctgaagcc gcctgtctgc acctcggtct ttttcacgat attcacttgg
2761 ggcatgctca gcactttccg cacggtggca aaatcccggc ccttatccca cacgatctcc
2821 ccggtttcgc cgtttgtctc gatcagaggc cgcttccgga tctcgccgtt ggccagggta
2881 atctcggtct tgaaaaagtt catgatgttg ctgtagaaga agtacttggc ggtagccttg
2941 ccgatttcct gctcgctctt ggcgatcatc ttccgcacgt cgtacacctt gtagtcgccg
3001 tacacgaact cgctttccag cttagggtac tttttgatca gggcggttcc cacgacggcg
3061 ttcaggtagg cgtcgtgggc gtggtggtag ttgttgatct cgcgcacttt gtaaaactgg
3121 aaatccttcc ggaaatcgga caccagcttg gacttcaggg tgatcacttt cacttcccgg
3181 atcagcttgt cattctcgtc gtacttagtg ttcatccggg agtccaggat ctgtgccacg
3241 tgctttgtga tctgccgggt ttccaccagc tgtctcttga tgaagccggc cttatccagt
3301 tcgctcaggc cgcctctctc ggccttggtc agattgtcga actttctctg ggtaatcagc
3361 ttggcgttca gcagctgccg ccagtagttc ttcatcttct tcacgacctc ttcggagggc
3421 acgttgtcgc tcttgccccg gttcttgtcg cttctggtca gcaccttgtt gtcgatggag
3481 tcgtccttca gaaagctctg aggcacgata tggtccacat cgtagtcgga cagccggttg
3541 atgtccagtt cctggtccac gtacatatcc cgcccattct gcaggtagta caggtacagc
3601 ttctcgttct gcagctgggt gttttccacg gggtgttctt tcaggatctg gctgcccagc
3661 tctttgatgc cctcttcgat ccgcttcatt ctctcgcggc tgttcttctg tcccttctgg
3721 gtggtctggt tctctctggc catttcgatc acgatgttct cgggcttgtg ccggcccatc
3781 actttcacga gctcgtccac caccttcact gtctgcagga tgcccttctt aatggcgggg
3841 ctgccggcca gattggcaat gtgctcgtgc aggctatcgc cctggccgga cacctgggct
3901 ttctggatgt cctctttaaa ggtcaggctg tcgtcgtgga tcagctgcat gaagtttctg
3961 ttggcgaagc cgtcggactt caggaaatcc aggattgtct tgccggactg cttgtcccgg
4021 atgccgttga tcagcttccg gctcagcctg ccccagccgg tgtatctccg ccgcttcagc
4081 tgcttcatca ctttgtcgtc gaacaggtgg gcataggttt tcagccgttc ctcgatcatc
4141 tctctgtcct caaacagtgt cagggtcagc acgatatctt ccagaatgtc ctcgttttcc
4201 tcattgtcca ggaagtcctt gtccttgata attttcagca gatcgtggta tgtgcccagg
4261 gaggcgttga accgatcttc cacgccggag atttccacgg agtcgaagca ctcgattttc
4321 ttgaagtagt cctctttcag ctgcttcacg gtcactttcc ggttggtctt gaacagcagg
4381 tccacgatgg cctttttctg ctcgccgctc aggaaggcgg gctttctcat tccctcggtc
4441 acgtatttca ctttggtcag ctcgttatac acggtgaagt actcgtacag caggctgtgc
4501 ttgggcagca ccttctcgtt gggcaggttc ttatcgaagt tggtcatccg ctcgatgaag
4561 ctctgggcgg aagcgccctt gtccaccact tcctcgaagt tccagggggt gatggtttcc
4621 tcgctctttc tggtcatcca ggcgaatctg ctgtttcccc tggccagagg gcccacgtag
4681 taggggatgc ggaaggtcag gatcttctcg atcttttccc ggttgtcctt caggaatggg
4741 taaaaatctt cctgccgccg cagaatggcg tgcagctctc ccaggtggat ctggtggggg
4801 atgctgccgt tgtcgaaggt ccgctgcttc cgcagcaggt cctctctgtt cagcttcacg
4861 agcagttcct cggtgccgtc catcttttcc aggatgggct tgatgaactt gtagaactct
4921 tcctggctgg ctccgccgtc aatgtagccg gcgtagccgt tcttgctctg gtcgaagaaa
4981 atctctttgt acttctcagg cagctgctgc cgcacgagag ctttcagcag ggtcaggtcc
5041 tggtggtgct cgtcgtatct cttgatcata gaggcgctca ggggggcctt ggtgatctcg
5101 gtgttcactc tcaggatgtc gctcagcagg atggcgtcgg acaggttctt ggcggccaga
5161 aacaggtcgg cgtactggtc gccgatctgg gccagcaggt tgtccaggtc gtcgtcgtag
5221 gtgtccttgc tcagctgcag tttggcatcc tcggccaggt cgaagttgct cttgaagttg
5281 ggggtcaggc ccaggctcag ggcaatcagg tttccgaaca ggccattctt cttctcgccg
5341 ggcagctggg cgatcagatt ttccagccgt ctgctcttgc tcagtctggc agacaggatg
5401 gccttggcgt ccacgccgct ggcgttgatg gggttttcct cgaacagctg gttgtaggtc
5461 tgcaccagct ggatgaacag cttgtccacg tcgctgttgt cggggttcag gtcgccctcg
5521 atcaggaagt ggccccggaa cttgatcatg tgggccaggg ccagatagat cagccgcagg
5581 tcggccttgt cggtgctgtc caccagtttc tttctcaggt ggtagatggt ggggtacttc
5641 tcgtggtagg ccacctcgtc cacgatgttg ccgaagatgg ggtgccgctc gtgcttctta
5701 tcctcttcca ccaggaagga ctcttccagt ctgtggaaga agctgtcgtc caccttggcc
5761 atctcgttgc tgaagatctc ttgcagatag cagatccggt tcttccgtct ggtgtatctt
5821 cttctggcgg ttctcttcag ccgggtggcc tcggctgttt cgccgctgtc gaacagcagg
5881 gctccgatca ggttcttctt gatgctgtgc cggtcggtgt tgcccagcac cttgaatttc
5941 ttgctgggca ccttgtactc gtcggtgatc acggcccagc ccacagagtt ggtgccgatg
6001 gccaggccga tgctgtactt cttgtcgctg ccgccgctgc tgccgccact gctctctggt
6061 gttgctgact cgcttgttcc tggtgtctcg ctgccagagg atcctccgct agatcctcca
6121 gacttcaggc ctgtggccca caggatgtgg ggtggcagcc tctgatagtg acaagactgc
6181 agggcgattg taaagaaggt cagctggggc tgctttctcc gcaggatatt cagacaaggg
6241 ggcaggccca ggatgatgca atacagctcc agcacgtaca ggcgcaccca caggtgtggg
6301 tacctaggcc agtgggcctc gttgctagga gaataattca cgaagttccg ccagcagtag
6361 ccggactcct gctctgtcat gatctggatg gtcacgccgg agctgatcag atcgcgcagg
6421 ccctgcctat tccttgggtc tgcgtggtga tacagcctgg cgatgtagat aaacagtgtc
6481 acgtgtggat atctggacag gaactcggtg atggccctgc tacactctcc gcaaggggac
6541 cagctcagaa accatgtgat gctacatctg gtattggggc agaagtaccg ctctgtggta
6601 aacttctcga tgaagttcac ctccacgtgc ttatttgtgt tctgagaggt gtgcctccag
6661 atggagtgcc tgcctcccca gttgatctcg tacagcaggc atgtctcctt ggccagctcc
6721 cgggggtcaa agaacacttc aaactcgtga ggttcaatcc ggcggcgcag ggttggatcg
6781 acggcgacag gcccagtctc tgaggagctc cccccgctcc cgccgctccc gccgctctcg
6841 ctctcggcgg gcaacacggg catccagtca atgggggtct cgccccgttg ttccagccac
6901 tgattggcca gcacgaaatg gttgcagccg aagaagcccc ggtgggcgct aagggggctg
6961 gggtgggggg ctttcagcac atgatgccgc tgcttgtcga tgatggcgcc tttcttctgg
7021 gcatggctgc cccacagcag aaacaccacg ccctcccggt gctggttgat caggctgatc
7081 accttatcgg tgaaggtctc ccagcccagg ctggcgtggc tatgcgcctg gccggcccgt
7141 accgtcagca cggtgttgag cagcagcacg ccctgccgcg cccagctctc caggtagccg
7201 tgattgggcc gggtgaagcc ggggatggtg ttttccagct ccttatacat gttcagcagg
7261 ctggggggga tggcgatgcc gggccgcacg ctgaaggcca gaccatgggc ctggccgggg
7321 ccgtggtagg ggtcctggcc caggatcacc accttcacgt cgcccagctc tgtgaagcgg
7381 aaggcgttaa acacgtcttt ctgtgggggg tagatggtca cgccgctctg ccgctcgctg
7441 gccacggtct gcagggtgtt aaggaaatag ggctgctgct tctcttcggc cagcacgtcg
7501 tgccaggtca gttcgttggc gactttccgc ttcttctttg gtgactcgaa ctcgcttccg
7561 tcggctgtcc gtttcatggt ggcggctctc cctatagtga gtcgtattag cggccgcgga
7621 tctctagcgg atctgacggt tcactaaacc agctctgctt atatagacct cccaccgtac
7681 acgcctaccg cccatttgcg tcaatggggc ggagttgtta cgacattttg gaaagtcccg
7741 ttgattttgg tgccaaaaca aactcccatt gacgtcaatg gggtggagac ttggaaatcc
7801 ccgtgagtca aaccgctatc cacgcccatt gatgtactgc caaaaccgca tcaccatggt
7861 aatagcgatg actaatacgt agatgtactg ccaagtagga aagtcccata aggtcatgta
7921 ctgggcataa tgccaggcgg gccatttacc gtcattgacg tcaatagggg gcgtacttgg
7981 catatgatac acttgatgta ctgccaagtg ggcagtttac cgtaaatact ccacccattg
8041 acgtcaatgg aaagtcccta ttggcgttac tatgggaaca tacgtcatta ttgacgtcaa
8101 tgggcggggg tcgttgggcg gtcagccagg cgggccattt accgtaagtt atgtaacgcg
8161 gaactccata tatgggctat gaactaatga ccccgtaatt gattactatt aataactagt
8221 caataatcaa tgtcaacgcg tatatctggc ccgtacatcg cgaagcagcg caaaacgcct
8281 aaccctaagc agattcttca tgcaattgtc ggtcaagcct tgccttgttg tagcttaaat
8341 tttgctcgcg cactactcag cgacctccaa cacacaagca gggagcagat actggcttaa
8401 ctatgcggca tcagagcaga ttgtactgag agtcgaccct aggggatcgg gagatcgatc
8461 tcccgatccg tcgacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
8521 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
8581 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
8641 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
8701 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
8761 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
8821 agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg
8881 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
8941 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
9001 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
9061 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
9121 accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact
9181 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
9241 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
9301 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg
9361 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
9421 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca
9481 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
9541 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
9601 ctgagtgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
9661 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
9721 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
9781 tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
9841 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
9901 tcttaccgg
//
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